Where does the SK-hep-1 cell line come from?
Where does the SK-hep-1 cell line come from?
An alternative hypothesis regarding the origin of the SK-HEP-1 cells was presented by Heffelfinger and colleagues, who claim that these cells do not display properties of hepatocytes and are of endothelial origin (PubMed ID: 1371504 ). This cell line may be licensed nonexclusively for research or commercial purposes.
How to grow HepG2 and SK HEP1 cells?
HepG2 and SK-Hep1 cells (1 x 10 3 cells) were treated with various concentrations (0, 7 and 8.5 μM) of BK10007S for 24 h and culture media were changed every three days. Cells were incubated for 2 weeks to grow colonies at 37°C under the condition of 5% CO2, fixed with methanol, stained with crystal violet solution and dried overnight.
How does ISX affect the growth of SK HEP1 cells?
Figure Legend Snippet: ISX enhances E2F1-mediated cell proliferation and oncogenic activity A. Effect of ISX and E2F1 on the cell growth of SK-Hep1 cells transfected with forced expression vectors of ISX-GFP and/or E2F1-GFP and knockdown vector of ISX and E2F1 . SK-Hep1 cells expressed endogenous ISX. a, p
An alternative hypothesis regarding the origin of the SK-HEP-1 cells was presented by Heffelfinger and colleagues, who claim that these cells do not display properties of hepatocytes and are of endothelial origin (PubMed ID: 1371504 ). This cell line may be licensed nonexclusively for research or commercial purposes.
HepG2 and SK-Hep1 cells (1 x 10 3 cells) were treated with various concentrations (0, 7 and 8.5 μM) of BK10007S for 24 h and culture media were changed every three days. Cells were incubated for 2 weeks to grow colonies at 37°C under the condition of 5% CO2, fixed with methanol, stained with crystal violet solution and dried overnight.
Figure Legend Snippet: ISX enhances E2F1-mediated cell proliferation and oncogenic activity A. Effect of ISX and E2F1 on the cell growth of SK-Hep1 cells transfected with forced expression vectors of ISX-GFP and/or E2F1-GFP and knockdown vector of ISX and E2F1 . SK-Hep1 cells expressed endogenous ISX. a, p
